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on the basis of their mechanism of action, antibiotics are classified as 1 those that affect bacterial cell-wall biosynthesis, causing loss of viability and often cell lysis penicillins and cephalosporins, bacitracin, cycloserine, vancomycin ; 2 those that act directly on the cell membrane, affecting its barrier function and leading to leakage of intracellular components polymyxin ; 3 those that interfere with protein biosynthesis chloramphenicol, tetracyclines, erythromycin, spectinomycin, streptomycin, gentamycin ; 4 those that affect nucleic acid biosynthesis rifampicin, novobiocin, quinolones ; and 5 those that block specific steps in intermediary metabolism sulfonamides, trimethoprim .
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